Search results for "Laboratory methods"
showing 7 items of 7 documents
Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.
2017
We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratorie…
Die acidolytische depolymerisation von trioxan in stationärer phase. 21. Mitt. über polyoxymethylene1
1964
Die Depolymerisation von Trioxan mit verschiedenen Phosphorsauren wurde im Temperaturbereich von 100–160°C untersucht. Sie fuhrt zu einem temperaturabhangigen Gleichgewicht zwischen Trioxan und Formaldehyd, das oberhalb 180°C weitgehend auf der Seite des Formaldehyds liegt. Zu einer schnellen Einstellung des Gleichgewichtes eignen sich Ortho- und Pyrophosphorsaure. Die bevorzugte Nebenreaktion ist die Bildung von Methylformiat; die Ausbeute an Methylformiat wird durch Erhohung der Reaktionszeit und der Katalysatoroberflache vergrosert. Das geschilderte Verfahren eignet sich zur Darstellung von monomerem Formaldehyd (Reinheitsgrad etwa 99,8%) im Laboratorium. Depolymerization of trioxane by …
Seeking the Holy Grail: robust chronologies from archaeology and radiocarbon dating combined
2018
The strengths of formal Bayesian chronological modelling are restated, combining as it does knowledge of the archaeology with the radiocarbon dating of carefully chosen samples of known taphonomy in association with diagnostic material culture. The risks of dating bone samples are reviewed, along with a brief history of the development of approaches to the radiocarbon dating of bone. In reply to Strien (2017), selected topics concerned with the emergence and aftermath of the LBK are discussed, as well as the early Vinča, Ražište and Hinkelstein sequences. The need for rigour in an approach which combines archaeology and radiocarbon dating is underlined.
El exilio en primera persona. Un análisis del film Journal Inachevé de Marilú Mallet / Exile in the first person. An analysis of Journal Inachevé by …
2016
Este artículo estudia el proceso de recuperación de la cultura gallega en el exilio, la creación del proyecto empresarial del Laboratorio de Formas y la pervivencia del mismo, desde su puesta en marcha durante el franquismo hasta la democracia. Se aborda el importante papel que desempeñó para la recuperación de la memoria histórica gallega y se hace referencia a la difícil situación que está protagonizando. Palabras clave: Exilio, Patrimonio, Políticas de la Memoria, Cultura, Democracia. This article studies the process of recovery of Galician culture in exile, the creation of business project Laboratory methods and its survival, since Franco's dictatorship regime to democracy. It's an impo…
Rheological Evaluation of Some Laboratory Mills
2012
The dough rheology is one of the most important aspect in bread making so it is very important to accurate evaluate the wheat potential for breadmaking by rheological test. The wheat must be milled for testing; the mill used influence the relevancy of wheat tests. The objective of this study is to evaluate the rheological properties of flour obtained by grinding ten samples of wheat in three of the most common laboratory mills (Perten 120, Quadromat Jr. and Chopin CD1). The rheological behaviours of the wheat flours obtained in laboratory were compared with those of flours obtained in industrial mill. The rheological measurements were performed using the Alveograph, Farinograph and Mixolab.…
Two New Alternatives to the Conventional Arm-in-Cage Test for Assessing Topical Repellents
2021
Abstract European guidelines for testing attractant and repellent efficacy (i.e., Product type 19 [PT19]) have been in revision since 2017. A key topic of discussion is the current approach to evaluating topical repellents. The European Chemical Agency has stated field testing should be avoided because of mosquito-borne disease risks. However, the most common laboratory method, the arm-in-cage (AIC) test, may limit the reliable extrapolation of lab results to field conditions. This study’s main goal was to assess alternative laboratory methods for evaluating topical mosquito repellents that use mosquito landing rates more representative of those in the field. The study took place at three E…
Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
2020
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims…